Precision medicine depends on molecular measurements that are sensitive, multiplexed, and actionable on the timescale of clinical decision-making. Yet many genetic and epigenetic assays remain too slow, costly, or infrastructure-dependent to be used routinely at the point of care or in low-resource settings. In this talk, I will present micro/nano-enabled diagnostic systems that convert complex molecular assays into portable, affordable, sample-to-answer workflows.
The core strategy integrates three complementary elements: (i) digital partitioning in microfluidic arrays and droplets to detect rare targets, achieve single-molecule/single-cell sensitivity, and resolve biological heterogeneity; (ii) melt‑coded sensing as an information-rich transducer that enables compact multiplexing with simplified optical hardware; and (iii) magnetofluidic automation using magnetic beads to integrate nucleic acid extraction, washing, and amplification/detection in low-cost cartridges with portable instrumentation. I will highlight applications spanning two time-critical clinical needs: rapid pathogen identification with antimicrobial resistance testing to guide evidence-based therapy, and high-precision DNA methylation analysis for early cancer detection from scarce circulating DNA. Together, these examples illustrate how digitization, melt coding, and cartridge automation can help democratize precision diagnostics by bringing actionable molecular results closer to patients.